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KMID : 0881720230380050338
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Journal of Food Hygiene and Safety
2023 Volume.38 No. 5 p.338 ~ p.346
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Statistical Optimization of Culture Conditions for Lactobacillus Strains using Response Surface Methodology
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Hwang Young-Min
Lee Hee-Seok
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Abstract
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The demand for probiotic products has been steadily increasing, and Lactobacillus strains are widely used and are currently the most popular probiotics. Optimizing culture conditions for Lactobacillus production for use as probiotics will enhance their profitability by reducing production costs and time. Statistical analysis using response surface methodology revealed the following optimal sets of independent variables: 22.55 h (cultivation time), 25°C (cultivation temperature), and 3.41% (w/w, prebiotics concentration) for Lactobacillusacidophilusf; 24 h, 30.86°C, and 2% (w/w) for Lactiplantibacillus plantarum; 66.67 h, 35°C, and 3.41% (w/w) for Lacticaseibacillus rhamnosus. Actual outcomes using predicted optimal conditions for Lactobacillus strains have been confirmed to closely match predicted results. This study will provide valuable guidelines for high yield Lactobacillus production.
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KEYWORD
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Lactobacillus, Culture condition, Optimization, Response surface methodology
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